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CANADIAN CITIZEN

EDUCATION
1967-72:
BSc Physics
Honors ( Physics Applied to Med & Biol, Physiol & Biophys )
Sch Physics - Havana U
( Moscow State Univ Program )
1972-77:
PhD Biology ( Physics Applied to Med, Physiol & Biophys )
Thesis: The Sinus Node Function
Natl Ctr for Sci Invest
( CNIC )
Havana U
( USSR Acad Sci Program )

POST-DOCTORAL TRAINING
1977-78:
Natl Inst Cardiol Cardiovasc Surg, Havana
1979-83:
Dalhousie U, Dept Physiol & Biophys, Halifax, NS

POSITIONS HELD

2007-Present:
Director, The Nuclear Physiology Lab
The Millennium Institute of Nanosciences & Nanotechnology, Brazil

2003-07:
Prof Med Physics, Physiol & Biophys
Fed U & Univ Hospital
Depts Physics & Med
Sergipe, Brazil

2003-07:
Leader, Nano-Biotechnology & Medical Physics Groups
Univ Federal, SE, Brazil
Leader, Nano-Biotechnology Group
Braz Nanophoton Network-CNPq
Leader, Nano-Biotechnology Group,
Biotechnology Network of Sergipe, Brazil
Leader, Nano-Biotechnology Group
South-Amer Network of Nano-Biotech-CNPq
Leader, Nano-Biotechnology Group
Braz Network of Nanosci & Nanotech-CNPq
Leader, Nano-Biotechnology Group
Millenium Inst Nanosciencesl-CNPq

1998-03:
Prof & Director, Nuclear Physiology Lab
U Tiradentes, Hlth Sci Ctr, Aracaju, Brazil

1997-98:
Visit Prof, U São Paulo
Fac Med & U Hosp, Dept Physiol
Ribeirão Preto, São Paulo

1994-97:
Adjunct Prof, Memorial U
Fac Med-Htlh Sci Ctr, St John's, NFLD

1995-96:
Visiting Scientist
Yale U Sch Med & Med Ctr
Depts Med, Surg & Physiol, New Haven

1992-94:
Visiting Assoc Prof
U Maryland Sch Med-Med Ctr
Dept Med & Physiol, Baltimore

1991-92:
Assoc Prof
U Ottawa, Heart Inst & Dept Physiol

1989-91:
Assistant Prof
U Ottawa, Heart Inst-& Dept Physiol

1983-89:
Assistant Prof
Dalhousie U Fac Med
Dept Physiol & Biophys, U Hosp, Halifax

1979-83:
Post-Doctoral Fellow, ibid

1978-83 *** NB ***
Defection to Canada on 12/24/78

1977-78:
Assoc Scientist
Natl Inst Cardiol & Cardiovasc Surg
Dept Exptl Res, Havana

1972-77:
Junior Scientist-Graduate Student ibid

GRANTS & PERSONNEL AWARDS
Please, visit http://sail.to/bustamante for detailed information

1983-89:
Research Scholarship, Heart & Stroke Foundat Canada
1979-83:
Post-Doc Fellowship, idem
1979-83:
Post-Doc Fellowship, USSR Acad Sci (declined due to defection to Canada)

ADMINISTRATIVE AND TEACHING EXPERIENCE
Please, visit http://sail.to/bustamante for detailed information

US, Canada and Brazil
1983-05:
Member of Various Departmental and Institutional Committees.
Lab and Grant Administration

Brazil
1998-Present:
Grad and Under-Grad Courses in Med Physics, Med Physiol & Biophys, Nano-Biotech, Supervisor of Same.

US & Canada

1983-97:
Graduate Level (Medical, Nursing, Hlth Sci, Physics and Eng Phys Students):
Other:
.Consultant in Membrane Physiology, Biophysics and Pharmacology
.PhD, MSc Training/Supervision

1972-77:
Graduate Level (Medical and Nursing Students) - Selected Student Teacher:

1965-72:
Undergraduate Level (Physics and Life Sciences Students) - Selected Student Teacher:

SKILLS OF GENERAL INTEREST

Cell Culture:

Operation and Management of Cell/Tissue Culture Facilities (Cell Separation and Culture, Preparation of Special Media for Stem Cells and Immortalized Cells, etc.)

Genetic Engineering:

Operation and Management of Genetic Engineering Facilities (DNA Transfection and Other Types of DNA Transference, Development of Special Purpose Techniques)

Tissue Engineering:

Operation and Management of Tissue Bioengineering Facilities (Scaffold Tissue Growth and Special Purpose Techniques)

Computers:

Operation and programming (from super- to micro-computers since 1970. (Computers used include those from Cray, IBM, DEC, Xerox, IRIS and NEC).

Computer Operating systems:

Unix, VMS, MS-DOS, and Windows (X, 9x, Me, 2000, XP, Vista).

Computer Programming Languages:

Assembler, FORTRAN, BASIC and C.

Networking:

Local Area Networks, Microsoft and Netware, Internet, Intranet, etc.

Advanced Data Acquisition and Processing:
Specialized systems (Array Processors, Atomic & Nuclear Physics' CAMAC, GPIB, SCSI, etc).

System Integration:

Design and assembly of special-purpose computer systems.

E-Learning:

Computer-Assisted Instruction, Internet-Based and Distance Learning, etc

Image Processing and 3D-Reconstruction:
IDL, ENVI, PV-WAVE, Exponent Graphics, Matlab, Metamorph, Volocity, etc.

Databases:

Access, Excel, Lotus 1-2-3, Office 2007 (12)

Data Processing, Statistical Packages and Presentation:

MatLab, Origin, SigmaPlot, SPSS, pClamp, Powerpoint, Corel, Virtual Reality, Adobe-Macromedia Flash, Dimension, etc.

Medical & Bio-Medical Electronics:

Design and construction of ultra-sensitive systems for the recording of single ion channel signals from cells and organelles thereof. Mainly, patch-clamp, voltage-clamp, microelectrode instrumentation amplifiers, etc.
Design and construction of catheter electrodes for mapping the living heart conduction system during catheterization procedures.
Design and construction of multiple electrode systems for in situ mapping of cardiac electrical waves during open-chest surgery.
Design and construction of defibrillators, electrical recorders, etc.

Electro-Optics:

Design and construction of micro- and nano-manipulation integrated with light microscope systems for cell and organelle handling, inverted microscopes with increased optical gain.
Video-Inverted microscopes with increased optical gain for enhanced imaging.
Digital/deconvolution confocal systems for optical slicing of cells and organelles.

Nano-/Micro-Manipulation:

Design and construction of systems for nano- manipulation/imaging: scanning tunneling and atomic force microscopes. These instruments include systems for protection against environmental micro-vibration. This experience has permitted the unique possibility of maintaining patch-clamp recordings for up to 3 days in living cells and organelles. This facilitates studies of the mechanisms controlling gene activity and expression.

Lasers:

Design and construction of laser-based microsurgical equipment: integration of pulsed lasers with highly specialized reflecting microscope to focus laser rays.
Design and optimization of laser-scanning confocal microscopes.

Drawing, Drafting, Animation:

Design and production of illustrations, animations, etc.

Instruments Operation:

Patch-clamp, voltage-clamp and microelectrode amplifiers. Digital and analog chart recorders, oscilloscopes, etc. Pulsed, CW, frequency-tuned lasers. Laser-scanning and digital confocal microscopes. Nuclear and atomic physics instrumentation, X-ray crystallography. Field-emission scanning electron microscopes as well as conventional scanning and transmission electron microscopes. Scanning tunneling and scanning atomic force microscopes. Analytical balances and centrifuges, HPLC, RIA, electrophoresis equipment, advanced audio-visual equipment: Computer-based photographic/video cameras, computer-based equipment for slide production, etc. Medical Physics and Biomedical Equipment: MRI, Ultrasound, Defibrillators, etc.

PUBLICATIONS - PAPERS
JOB = JO BUSTAMANTE
1. FD, JOB et al. 1978. Effects of atrial premature stimulation on sinus node function in isolated rabbit atria. Cor et Vasa 20(5-6): 417
2. ED, JA, JM, FD & JOB. 1979. Rate-dependent action potential changes in rat atrium. J Physiol (Paris) 75(7): 729
3. JOB et al. 1981. Single cells from adult mammalian heart: Isolation procedure and preliminary electrophysiological studies. Can J Physiol Pharmacol 59(8): 907
4. JOB et al. 1982. Non-specific proteases: A new approach to the isolation of adult cardiocytes. Can J Physiol Pharmacol 60(7): 997
5. JOB et al. 1982. Isolation of single atrial and ventricular cells from the human heart. Can Med Ass J 126(7): 791
6. JOB & TFMcD. 1983. Sodium currents in segments of human heart cells. Science 220(4594): 320
7. TW, LMD, JOB & TFMcD. 1983. Heterogeneity of the action potential in isolated rat ventricular myocytes and tissue. Circ Res 52(3): 280
8. JOB. 1985. Block of sodium currents by the calcium antagonist D600 in human heart cell segments. Pflügers Archiv - Eur J Physiol 403(2): 225
9. JOB. 1987. Modification of sodium channel currents by lanthanum and lanthanide ions in human heart cells. Can J Physiol Pharmacol 65(4): 591
10. JOB. 1988. A system for the automated data-acquisition of fast transient signals in excitable membranes. Internat J Bio-Med Comput 22(3-4): 273
11. JOB & DJ. 1988. Cryopreservation of human heart cells. Cryobiology 25: 394
12. JOB. 1990. Depression of cardiac sodium conductance by atrial natriuretic peptide. In: Diuretics III: Chemistry, Pharmacology, and Clinical Applications. Eds. J.B. Puschett and A. Greenberg. Elsevier, New York, p: 589
13. JOB et al. 1991. Stretch-activated channels in heart cells: Relevance to cardiac hypertrophy. J Cardiovasc Pharmacol 17(s 2): S110
14. JOB. 1991. An inexpensive inverted microscope for patch-clamp and other electrophysiological studies at the cellular level. Pflügers Archiv-Eur J Physiol 418(6): 608
15. JOB. 1992. Nuclear ion channels in cardiac myocytes. Pflügers Archiv-Eur J Physiol 421(5): 473
16. AR, FS & JOB. 1993. Stretch-activated channels of chick cardiac myocytes. Am J Physiol 264(3 pt 2): H960
17. JOB. 1993. Restricted ion flow at the nuclear envelope of cardiac myocytes. Biophys J 64(6): 1735
18. JOB. 1994. Open states of nuclear envelope ion channels in cardiac myocytes. J Membr Biol 138(1): 77
19. JOB. 1994. Nuclear electrophysiology. J Membr Biol 138(2): 105
20. JOB et al. 1994. Nuclear pore complex ion channels. Mol Membr Biol 11(3):141
21. A Liepins & JOB. 1994. Cell injury and apoptosis. Scann Microsc 8(3): 631
22. JOB et al. 1995. The nuclear pore ion channel activity. J Membr Biol 146(3): 239
23. JOB et al. 1995. Patch-clamp detection of transcription factor translocation across nuclear pores. J Membr Biol 146(3): 253
24. JOB et al. 1995. Patch-clamp and atomic force microscopy demonstrate TATA-binding protein (TBP) interactions with the nuclear pore. J Membr Biol 146(3): 263
25. HO, SS & JOB. 1996. Atomic force microscopy visualizes ATP dependent dissociation of multimeric TATA-binding protein before translocation into the cell nucleus Pflügers Archiv-Eur J Physiol 432(5): 839
26. AL, JWN, JOB & EL. 1996. Induction of bimodal programmed cell death in malignant cells by the derivative Ukrain (NSC-631570). Drugs Under Exptl Clin Res 22(3-5): 73
27. JOB & WAV. 1998. Patch-clamp measurement of macromolecular translocation along nuclear pores. Brazil J Med Biol Res 31(3): 333
28. JOB et al. 2000. Calcium, ATP, and nuclear pore gating. Pflügers Arch - Eur J Physiol 439(4): 433
29. JOB et al. 2000. Dendrimer-assisted patch-clamp sizing of nuclear pores. Pflügers Arch - Eur J Physiol 439(4): 829
30. MM, JOB & HO. 2001. Electrical dimension of the nuclear envelope. Physiol Rev 81(1): 1
31. JOB. 2001-2008. Nuclear transport methods. In: Nuclear Import and Export In Plants and Animals. Eds. V.H. Citovsky & T. Tzvira. Landes Bioscience/Eureka.Com (Hardcopy and Internet)
32. JOB. 2002. Nuclear pore ion channel activity in living nuclei. Pflügers Archiv - Eur J Physiol 444(1): 286
33. JOB. 2002-2008. Recent advances in nuclear electrophysiology. Biophysical Textbook On-Line. On-Line. http://www.biophysics.org/btol. Ion Channels Section. Ed LJ DeFelice. Biophys Soc, Bethesda, MD
34. JOB. 2004. New biotechnological applications of coconuts. Electronic J Biotechnol 7: 1-4. http://ejbiotechnology.info/content/vol7/issue1/issues/1/index.html
35. JOB. 2005. Recent advances in nuclear electrophysiology. In: Educational Resources of the Biophys Soc USA. On-Line. http://www.biophysics.org/education/bustamante.pdf Ed. LJ DeFelice. Biophysical Soc, Bethesda, MD
36. JOB. 2006. Current concepts in nuclear pore electrophysiology. Can J Physiol Pharmacol 84(3-4): 347
37. JOB. 2008. Invited Review in preparation for a special section (Made-In-Canada) of the Can J Physiol Pharmacol (in preparation)
38. JOB. 2008. Invited Book in preparation: Nuclear Electrophysiology (Landes Biosciences) (in preparation).
PUBLICATIONS - SELECTED ABSTRACTS
1. FD, JOB, JA & JM. 1975. Re-entry in the rabbit right atrium. Proc XII Interamer Congr Physiol Sci, Bogotá, Colombia. Acta Physiol Latinoamer 25(s 4): 55
2. FD, JOB, JA & JM. 1975. Effects of premature atrial depolarizations on rabbit sinus node function. Ibid
3. FD, JOB, JM & JA. 1976. Effects of high rates of stimulation on the rabbit sinus node function. Ibid. p 56
4. ___. 1977. Effects of premature depolarizations and high stimulation rates on the rabbit sinus node function. Proc X Interamer Congr Cardiol. Caracas. Arch Venezol Cardiol 3: 76
5. JOB et al. 1977. Physical-mathematical model of the sinus node function. Ibid. p 78
6. JOB et al. 1977. Biophysics of the sinus node function. Proc XIII Interamer Congr Physiol Sci, México City
7. JA, JM, FD & JOB. 1977. Effects of hypoxia on the action potential of ventricular papillary muscle. Ibid
8. ___. 1977. Efects of glucose on the action potential of hypoxic ventricular papillary muscle. Ibid
9. JM, FD, JA & JOB. 1977. Effects of hyaluronidase on the guinea pig ventricular action potential. Ibid
10. JM, JA, JOB, FD, NG & JT, 1977. Repolarization in the human atrial action potentials at different stimulation rates. Ibid
11. JOB & TFMcD. 1980. A simple technique for isolating single adult heart cells with normal electrophysiological properties. Proc 23rd Annu Meet Can Fed Biol Soc St John's, Nfld. Can Physiol 10(3): 315
12. JOB & TFMcD. 1980. Simple method for the isolation of single adult heart cells with normal electrophysiological properties. Proc 13th Winter Meet Can Physiol Soc. St Adele, Quebec, Canada. Can Physiol 11(3): 65
13. JOB. 1981. Internal dialysis-induced alterations of the action potential recorded from single adult cardiocytes. Proc VII Internat Biophys Congr. México City p 334
14. JOB et al. 1981. Perfusion-voltage-clamp of human cardiocytes. Proc 14th Wint Meet Can Physiol Soc. Beaupré, Québec. Can Physiol 12(3): 98
15. TW, LMD, JOB & TFMcD. 1981. A comparative study on cardiac action potentials of single ventricular cells and tissue preparations. Ibid p 159
16. JOB & TFMcD. 1982. Sodium currents in perfused human heart cell sections. Proc IV Annu Meet Amer Sect Internat Soc Heart Res. New Orleans J Molec Cell Cardiol 14(s. I): 26
17. JOB. 1983. Rate- and voltage-dependent block of cardiac and neuroblastoma sodium currents by D600. Proc 26th Annu Meet Can Fed Biol Soc, Ottawa p 69
18. JOB. 1985. Heart sodium channels. Biomed Pharmacother 39(2): 104
19. JOB. 1989. Effects of atrial natriuretic peptide on cardiac ionic channels. Proc Third Internat Confer Diuret. México City
20. JOB et al. 1990. Stretch-activated channels in heart cells: Relevance to Cardiac Hypertrophy. Proc Internat Symp Cardiov Hypertrophy. Ottawa
21. JOB et al. 1991. Presence of stretch-activated channels in freshly isolated adult rat heart cells. Proc UCLA Symp Cardiac Growth Hypertrophy. Keystone, CO. J Cell Biochem s15C: 167
22. PHB, MLdeB, AJdeB & JOB. 1991. Activation mode of the atrial natriuretic factor gene during P19 cardiac myogenesis. Ibid: 160
23. AR, JOB & FS. 1991. Stretch-activated channels of chick cardiac myocytes. Ibid: 197
24. PHB, MLdeB, AJdeB & JOB. 1991. Activation mode of the atrial natriuretic factor gene during P19 cardiac myogenesis. Proc 25th Annu Meet Fed Amer Soc Exper Biol. FASEB J 5(6): A1748
25. HO, SS & JOB. 1995. Atomic force microscopy demonstrates that ATP splits multimeric transcription factor before translocation into the cell nucleus. Proc Winter Meet German Physiol Soc. Münster.
26. JOB & AL. 1995. Nuclear pore-mediated apoptosis. Scann Microsc Meet. Houston, TX
27. AL & JOB. 1995. T-cell induction of programmed cell death in malignant cancer cells. Scann Microsc. Meet. Houston, TX
28. JOB et al. 1995. Macromolecular Translocation Reduces Ion Flow Along Nuclear Pores: Relevance To Measuring Transcriptional Control Mechanisms. Symposium: 'Organellar Ion Channels and Transporters'. Soc Gral Physiol, Woods Hole, MA. J Gen Physiol 105: 25-26a
29. JOB. 1995. Invited Speaker. Patch-clamp assessment of transcriptional signaling through nuclear pores. Internat Symp 'Molecular Basis of Cellular Communication'. German Physiol Soc. Würzburg. 100th Anniversary of Wilhem Röntgen´s Discovery of X Rays.
30. HO, SS & JOB. 1995. ATP-dependent multimers of TATA-binding protein imaged with atomic force microscopy. Internat Symp 'Molecular Basis of Cellular Communication'. German Physiol Soc. Würzburg.
31. JOB. 1997. Invited Speaker. Patch-clamp detection of transcription factor translocation. XXXIII Internat Congr Internat Union Physiol Sci: Satellite Symposium 'Nuclear Pore Function'. St. Petersburg, Russia. June 28-July 5.
32. JOB. 1997. Invited Speaker. Patch-clamp detection of transcription factor translocation. XII Ann Meet Braz Fed Biol Soc (FeSBE). Caxambu, MG, Brazil. Aug 27-30.
33. JOB. 1997. Invited Speaker. Effects of nuclear calcium on nuclear pore ion channel behaviour and nuclear transport of transcription factor and mRNA. First European Conference on Calcium Signalling in The Cell Nucleus. Baia Paraelios-Calabria, Italy. October 4-8.
34. JOB. 1998. Invited Speaker. Calcium, nuclear pore ion channel gating behavior and nuclear translocation of transcription factors and mRNAs. First EMBO Workshop on Calcium Signals in The Cell Nucleus. Strasbourg, France. August 20-23.
35. JOB. 2002. Syncytial nuclei for nuclear pore functional analysis and for protein and nucleic acid biotechnology. XI Braz Congr Cell Biol. Porto Alegre, Rio Grande do Sul, Brazil. July 16-19. http://www.pucrs.br/biocel
36. JOB. 2002. Invited Speaker. The molecular Coulter-counter principle for the detection and measurement of the translocation of DNA, RNA and other important macromolecules along nuclear pores. 3rd Int Conf Unsolved Problems of Noise and Fluctuations in Physics, Biology, and High Tech. NIH-Bethesda, MD. Sept 2-7. (cancelled for lack of funds).
37. JOB et al. 2003a. Atomic force microscopy imaging of nuclear pore assembly in syncytial nuclei and its relationship to nanopore permeability and conductance. V Ibero-Amer Congr Biophys. Rio de Janeiro, October 12-15, 2003
38. JOB et al. 2003b. Atomic force microscopy imaging of nuclear pore assembly in Drosophila melanogaster syncytial. XIX Congr Braz Soc Microsc Microanal, Caxambu, 21-24 Sept 2003.
39. JOB. 2004. New biotechnological applications of coconut water. Congress Redbio 2004, Santo Domingo, 22-25 June, 2004.
40. JOB. 2005a. Clinical importance of the voltage- and rate-dependent block of cardiac sodium channels by specific calcium antagonists. XX Ann Meet Federat Biol Soc Brazil (FeSBE). Águas de Lindoia, Sao Paulo, 24-27 August, 2005. Poster
41. JOB. 2005b. Laser-scanning confocal microscopy of living nuclei explains apparent paradox of nucleocytoplasmic ion gradients despite the large aqueous diameter of nuclear pores. XX Ann Meet FeSBE - Idem - Poster.
42. JOB. 2005. Invited Speaker. Electrical detection of macromolecular transport across the nuclear pore. XX Ann Meet FeSBE. Idem.
REVIEWER SERVICES FOR GRANTING AGENCIES (ca. 8) AND SCIENTIFIC JOURNALS (ca. 20)

OTHER ACTIVITIES
Guest Editor – Can J Physiol Pharmacol - 2005-2006.
Organizer of Internat Course on Nano-Biotechnol - Eur Molec Biol Org (EMBO) - 2006-2007

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Dynamic, hands-on international oilfield operations Finance & Administration Manager
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