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EDUCATION
* 2002 – 2004, School of Biosciences & Bioengineering, Indian Institute of Technology ( IIT ) Bombay, Powai, Mumbai – 400076, India
M.Sc. Biotechnology

* 1999 – 2002, Sri Venkateswara College, Delhi University, India
B.Sc.(Hons.) Biochemistry

* 1998 – 1999, Sahoday School, S.D.A, New Delhi, India
10 + 2, CBSE

OTHER QUALIFICATIONS
* Computer Knowledge
à September, 2000 – July, 2001, Diploma in computers from Wintech computers, New Delhi, India.
(MS Dos, MS Office, MS Access, Internet Applications, Html, FrontPage, Java Script, 'C' Programming, Oracle 9i).
à Working Knowledge of Perl, Java, 'C', XML, XSLT.
à Experience in working on Windows and Linux/Solaris Operating Systems

* Pursued a Lecture Series on "The Entrepreneurship Development Program" certified by E-cell, IIT Bombay.
* Pursued an Audit course in "Advertising and Media Management" at SJM School of Management, IIT Bombay.

EXPERIENCE
* July, 2004 - July, 2005 Persistent Systems Pvt. Ltd., Pune, India
Member of Technical Staff (Bioinformatics)

Job Responsibilities:
- Joined as 'Domain Consulant' in the team (Team Size – 7).
- Support and Enhancement of the following Bioinformatics products:
- Synapsia Informatics Workbench (Clinical Data Management Software).
- GeneSpring (Specialized Software for analysis of Gene Expression and Microarray data).
- Varia (Specialized Software for genotyping and Genetic Variation data analysis).
- SigNet (Data Repository)

- Contributed towards training other team members and the software development team on GeneSpring and Varia.
- Contributed towards developing Knowledge Base and FAQs for GeneSpring, Varia and Synapsia.
- Contributed to the interview and selection process of the right candidates for the project.

US Experience:
- Received extensive training on 'GeneSpring, Varia and Signet' from Agilent Technologies and Silicon genetics at California, US.
- Performed QA and Testing for Varia and GeneSpring.
- Contributed towards developing and updating the Training Modules and User Guides for Varia, GeneSpring and Synapsia.

* July, 2003 – May, 2004 IIT Bombay, Powai, Mumbai – 400076, IndiaOne-year project under the guidance of Dr. S.Durani and Dr. P.S.Phale at School of Biosciences and Bioengineering, IIT Bombay.
Project Title: Re-engineering of catechol 2,3-dioxygenase towards modified substrate specificity using Inverse Design (computational approach) and Site Directed Mutagenesis (experimental approach).

* May, 2003 – July, 2003 Jawaharlal Nehru University, Delhi, IndiaSummer project under the guidance of Dr. Sudha Bhattacharya and Dr. Alok Bhattacharya in Life Sciences department at Jawaharlal Nehru University.
Project Title: Sequence analysis of Short Interspersed non-LTR Retrotransposon (SINE) element using Entamoeba Genome Database to find the hotspot for its insertion.

* Laboratory Experience:
* Molecular Biology / Genetic Engineering Lab Techniques.
* Biochemistry Lab Techniques
* Biomolecular Spectroscopy and Biophysical Lab Techniques
* Microbiology Lab Techniques
* Enzymology and Protein Biochemistry Lab Techniques
* Bioinformatics and Computational Biology

Bioinformatics Skills Databases:
Nucleotide GenBank, EMBL, DDBJ, Ensembl, Contig, dbSNP
Protein SwissProt, PIR, Pfam, PROSITE, BLOCKS, PRINTS, Interpro, DSSP, SCOP, CATH
Others COG, KEGG, BRENDA, OMIM, Entrez(NCBI)

Sequence Analysis Software: BLAST suite, FASTA, CLUSTALW
Structure Analysis Software: DS Modeller, Rasmol, Spdbv, Swiss-Model, 3D-PSSM, GROMACS, Cn-3D
Microarray / Gene Expression Data Analysis: GeneSpring and Signet
Genotyping, Variation and Pedigree Analysis: Varia
Statictical Packages: R Package

Extracurricular Activities
* Joint Secretary of Symbiotek (a non academic organization of Biotechnology group, IIT Bombay).
* Volunteer organizer of Generations (a quiz and poster competition on Biotechnology organized by Symbiotek, IIT Bombay).
* Volunteer coordinator in the marketing division of MoodIndigo (the annual cultural festival of IIT Bombay).
* Hostel representative in Inter hostel athletic events at IIT Bombay.Member of the student body council at the school level.

Summer Jobs
Worked as the promoter and the marketing coordinator for the following brands:
- Colgate
- Laughing cow cheese.
- Dr. Morepen

Other Information
* CSIR-UGC-NET qualified, eligible for Lectureship at any Indian University.
* Holds a US Business visa valid till 2014.
* Ranked 203 in Mumbai International Marathon 2005.

References
Available upon request

Annexure I

Project Details:

* July, 2003 – May, 2004 IIT Bombay, Powai, Mumbai – 400076One year project under the guidance of Dr. S.Durani and Dr. P.S.Phale at School of Biosciences and Bioengineering, IIT Bombay. Project Title: Re-engineering of Catechol 2,3 Dioxygenase towards modified substrate specificity using Inverse Design (computational approach) and Site Directed Mutagenesis (experimental approach). The main objective of the project was to re-engineer the enzyme 'catechol 2,3-dioxygenase' to accept a wider variety of substrates without compromising much on its catalytic efficiency. Catechol 2,3-dioxygenase is a key enzyme catalyzing the extradiol ring cleavage of catechol and its derivatives, which are intermediates in the biodegradation of a number of polyaromatic hydrocarbons toxic to the environment. To achieve this objective a rational approach involving the coherent use of both the computational and experimental strategies was followed. The computational approach involved implementation of various in-house built and online available bioinformatics tools and algorithms for generating a number of artificial ligands and individual docking of each ligand at the active site of the enzyme. This was followed by mutating the residues whose side chain atoms were stearically clashing with the atoms of the newly generated ligand. The mutation was performed using an in house built program that uses LJ energy and the solvation energy as the deciding parameters to select the possible rotamers from a rotamer library and generate the optimized sequence. To perform the Molecular Dynamics, GROMACS and its packages were extensively used.The experimental approach involved the implementation of a suitable cloning strategy so as to experimentally justify the results obtained through the computational method. This involved designing of suitable primers and the PCR amplification of the required gene from the organism (Pseudomonas) under study. This was followed by the cloning of the PCR amplified product into a suitable host using an appropriate vector system. The new modified enzyme was then tested for its modified substrate specificity.

* May, 2003 – July, 2003 Jawaharlal Nehru University, DelhiSummer project under the guidance of Dr. Sudha Bhattacharya and Dr. Alok Bhattacharya in Life Sciences department at Jawaharlal Nehru University.Project Title: Sequence analysis of Short Interspersed non-LTR Retrotransposon (SINE) element using Entamoeba Genome Database to find the hotspot for its insertion.The main objective of the project was to find the hotspot for the insertion of SINE element in order to interpret the behavior of the enzymes involved in this process of insertion. These SINE elements and their corresponding LINE elements are found to be interspersed as multiple copies in the genome of the organism they are present in. The objective was partially achieved by studying the pattern of the 5' and the 3' flanking regions around the already existing SINE elements in the Entamoeba genome using Entamoeba Genome Database available at NCBI. The study involved the implementation of a number of standard computational tools such as BLAST, ClustalW, BioEdit etc.

Annexure II

Courses pursued during M.Sc.

First Semester Second Semester
- Chemistry of Biomolecules è Molecular Genetics
- Analytical Biochemistry è Molecular Biology
- Fundamental Microbiology è Biomolecular Spectroscopy
- Chemical and Statistical Thermodynamics è Catalysis and Mechanisms
- Computational Biology è Rate processes
- Biochemistry Laboratory è Biophysics Laboratory
- Microbiology Laboratory

Third Semester Fourth Semester
- Molecular Enzymology è Molecular Biophysics
- Genetic Engineering è Cell Biology
- Molecular Immunology è Bioelectricity
- Metabolism and Bioenergetics è Bioremediation: Principles
- Bioinformatics and its Applications
- Genetic Engineering Laboratory è Project: Stage II
- Project: Stage I

Courses pursued during B.Sc.

First Year Second Year Third Year
- Physical Chemistry (+ Lab) è Biomolecules è Molecular Biology
- Inorganic and Organic è Biochemical and Biophysical è Amino acid & Nucleotide
Chemistry (+ Lab) Techniques Metabolism
- Physics (+ Lab) è Proteins and Enzymes è Cell Biology & Immunology
- Math and Statistics è Vitamins and Hormones è Bioenergetics & Membranes
- Introductory Biology (+ Lab) è Carbohydrate and Lipid Metabolism è Physiology
- Biochemistry Lab è Molecular Biology Lab è Enzyme Kinetics Lab è Microbiology Lab

Courses pursued during 10+2
- English
- Physics (Theory + Lab)
- Chemistry (Theory + Lab)
- Mathematics
- Biology (Theory + Lab)

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